THUNDER Phospho-BTK (Y223) + Total BTK TR-FRET Cell Signaling Assay Kit

Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-BTK (Y223) and Total BTK in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total BTK proteins from the same lysate sample in separate wells.

Components: Eu-phospho-BTK (20 µL), Acc-phospho-BTK (80 µL), Eu-Total BTK (5 µL), Acc-Total BTK (20 µL), BTK control lysate (500 µL), 5X Lysis Buffer #2 (5 mL), 10X TR-FRET Detection buffer (250 µL).

Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.

Assay Format: Half-area 96-well or low-volume 384-well plate.

Assay Target: Phospho-BTK (Y223) & Total BTK.

Assay Target Class: Kinase.

SwissProt: Q06187.

Detection method: TR-FRET.

Sample type: Cell lysates (adherent or suspension cells).

• SKU: BIXKIT-BTKPT-500
• Manufacturer: BioAuxilium
• Manufacturer SKU: KIT-BTKPT-500
• Green Labware: No
• Package Unit: 500 Points (400 Points for phospho; 100 Points for total)
• Quantity Unit: PAK
• Reactivity: Human
• Application: Cell-based Assay
• Human Gene ID: 695