PD-L1 Lentivirus

Products from BPS Bioscience require a minimum order value above 400€

Applications: Expression of human PD-L1 in cells of interest.Generate cell pools or stable cell lines expressing human PD-L1 following geneticin selection

Background: The binding of Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated T cells, to its ligands, PD-L1 and PD-L2, negatively regulates immune responses. PD-1 ligands are found in most cancers, and PD-1:PD-L1/2  interaction inhibits T cell activity and allows cancer cells to escape immune surveillance. The PD-1:PD-L1/2 pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes.

Description: PD-L1 Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses contain human PD-L1 (programmed death ligand 1) (NM_014143.4) driven by an EF1a promoter and a geneticin selection marker (Figure 1).

Figure 1. Schematic of the lenti-vector used to generate the PD-L1 Lentivirus.

Formulation: The lentivirus particles were produced in HEK293T cells in medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations by special request, for an additional fee.

Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.

Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ?10⁷ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Warnings: Avoid freeze/thaw cycles

Biosafety Level: BSL-2

• SKU: BPS78925
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 78925
• Package Unit: 500 µl x 2
• Quantity Unit: STK