Products from BPS Bioscience require a minimum order value above 400€
Application: 1. Transient expression of FcGRIIB in target cells.
2. Generation of stable cell line expressing FcGRIIB with Geneticin (G418) selection.
Description: Fc Gamma Receptor 2B (FcGR2B, FcγRIIB), also known as CD32B, is a "low affinity" receptor for Immunoglobulin G (IgG). FcGR2B is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. It is the predominant Fc receptor present on B cells. Crosslinking of antibodies by FcGR-expression cells can promote receptor clustering and increase downstream signaling. FcGR2B crosslinking is important for anti-TNFR receptor antibodies.
The FcGRIIB Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types mammalian cells, including primary and non-dividing cells. The particles contain a FcGRIIB gene (NM_004001.4) driven by a CMV promoter.
Formulation: The lentiviruses were produced from HEK293T cells in the medium containing 90% DMEM + 10% FBS.
Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥5 x 106 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Warnings: Biosafety: of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.
Biosafety Level: BSL-2
English
Deutsch
