Shelf life (days): 365.0
Formulation: 400 µl of CNBr-activated Sepharose 4B coupled to Diglycyl-Lysine Monoclonal Antibody (Clone GX41) (Item No. 21096)
Immunogen: Diglycine-modified histone peptide
Clone Designation: GX41
Cross Reactivity: + Diglycyl-lysine moieties
Formula Weight: 0.0
Notes: Ubiquitin remnant (K-ε-GG) is a diglycyl-lysine motif found in proteins subject to ubiquitination, a process for tagging proteins for proteasomal degradation that is disrupted in certain diseases.{65646,65647} During ubiquitination, ubiquitin is covalently bound to the ε-NH2 in lysine residues of the target protein via the C-terminal carboxyl group of ubiquitin, which is preceded by arginine-glycine-glycine (-RGG) residues.{65648} Ubiquitinated proteins can be detected, and enriched for, via antibodies toward the K-ε-GG motif resulting from this covalent bond.{65646,65649} Cayman's Ubiquitin Remnant (K-ε-GG) Immunoaffinity Sorbent is designed for immunoprecipitation (IP) of ubiquitin remnant (K-ε-GG) motif-containing proteins from biological samples. This is an effective way to concentrate ubiquitin remnant (K-ε-GG) motif-containing proteins for subsequent detection and analysis by LC-MS/MS. The ubiquitin remnant (K-ε-GG) affinity sorbent consists of a diglycyl-lysine specific monoclonal antibody coupled to CNBr-activated Sepharose 4B.
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