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Applications: This product has been optimized for use with the BPS Bioscience Intrachain TR-FRET Assay Kits and U2 Assay Buffer (BPS Bioscience #78856). For a detailed protocol on the use of this product with BPS Bioscience Intrachain TR-FRET Assay Kits, please see the appropriate assay kit protocol. Use in other assays may require optimization.
Description: The 200x Ubi-Mix™ is a mix of Europium cryptate-labeled Ubiquitin (donor), Cy5-labeled Ubiquitin (acceptor), and unlabeled Ubiquitin at a ratio optimized for TR-FRET based assays where long and/or branched polyubiquitin chains are formed. Under conditions where short ubiquitin chains or multi-monoubiquitylation on a substrate protein are expected, a different reagent might be required.Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains, using 200x Ubi-Mix™ avoids time-consuming washing steps and allows for monitoring polyubiquitination real-time kinetics
Format: aqueous solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Mycoplasma Testing: The cells have been screened to confirm the absence of Mycoplasma species.
Storage Stability: Upon receipt, store at -80°C. Stable for 6 months from date of receipt, when stored as directed.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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