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Description: This kit contains four cell lysis buffers with varying detergent strengths (from mild to strong). This kit includes 20 ml of NP-40 Cell Lysis Buffer, Modified NP-40 Cell Lysis Buffer, Modified RIPA Lysis Buffer and RIPA Lysis Buffer. This kit is intended for optimization of mammalian cell lysis conditions that best suit the assay and protein of interest.
Formulation: NP-40 Cell Lysis Buffer (BPS Bioscience #82124):
50mM Tris HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1mM EGTA, 1% IGEPAL CA-630Modified NP-40 Cell Lysis Buffer (>BPS Bioscience #82125):
50mM Tris HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1mM EGTA, 1% IGEPAL CA-630, 0.1% sodium deoxycholateModified RIPA Lysis Buffer (BPS Bioscience #82126):
50mM Tris HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1mM EGTA, 0.5% Triton X-100, 0.5% sodium deoxycholate
RIPA Lysis Buffer (BPS Bioscience #82127):
50mM Tris HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1mM EGTA, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS
Mycoplasma Testing: Sterile filtered (0.2 µm).
Storage Stability: Upon receipt, store at 4°C. Stable for 6 months from date of receipt, when stored as directed.
Biosafety Level: Not applicable (BSL-1)
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