Type: Competitive.
Assay Time: 1.5h.
Detection Type: Colormetric.
Sensitivity: 9.38ng/mL.
Detection Range: 15.63~500ng/mL.
Target Synonym: COVID 19 Neutralization Antibody, Anti-RBD, RBD Antibody, S1RBD Antibody.
Tested Sample Types: Serum and plasma.
Test Principle: This ELISA kit uses Competitive-ELISA as the method to semi-quantitatively detect the Anti-SARS-CoV-2 Neutralization Antibody in the sample. The micro ELISA plate provided in this kit is pre-coated with recombinant human ACE2. During the reaction, the SARS-CoV-2 Neutralization Antibody in the pretreated samples or standards/controls competes with a fixed amount of human ACE2 on the solid phase supporter for sites on the Horseradish peroxidase (HRP) conjugated recombinant SARS-CoV-2 RBD fragment (HRP-RBD). After 37℃ incubation, the unbound HRP-RBD as well as any HRP-RBD bound to non-Neutralization antibody will be captured on the plate and eventually form the ACE2-RBD-HRP complex, while the circulating neutralization antibodies HRP-RBD complexes remain in the supernatant and are removed during washing. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SARS-CoV-2 Neutralization Antibody in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Areas: SARS-CoV-2, COVID 19
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