Conjugation: Unconjugated.
Description: The SARS-CoV-2 Spike Receptor Binding Domain N501Y (UK Variant Alpha) Recombinant Protein (OPSN00004) is expressed in Sf9 Baculovirus cells and includes a C-terminal Avidin-His Tag with purification via IMAC. The sequence includes amino acids 319-357 of the lineage B.1.1.7 VUI-202012/01 SARS-CoV-2 spike sequence first identified in the United Kingdom. Suitable for Biotinylation and Protein:Protein Interaction assays in a variety of formats.
Description of Target: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.
Key Reference: A. Rambaut et al., Preliminary genomic characterisation of an emergent SARS-CoV-2 lineage in the UK defined by a novel set of spike mutations (2020), (available at https://virological.org/t/preliminary-genomic-characterisation-of-an-emergent-sars-cov-2-lineage-in-the-uk-defined-by-a-novel-set-of-spike-mutations/563). Sci Rep. 2021 Apr 28;11(1):9166. doi: 10.1038/s41598-021-88696-5.
Molecular Weight: 30kDa.
Product Format: Liquid. 50 mM Tris-HCL, 1M NaCl, pH 7.5.
Protein Name: surface glycoprotein.
Protein Size: AA 319-537, N501Y (UK Variant Alpha).
Purification: IMAC.
Purity: 0,9.
Tag: C-Avitag/His
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