Target Synonym: SCAF8;RBM16
Storage: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Background: Anti-terminator protein required to prevent early mRNA termination during transcription. Together with SCAF4, acts by suppressing the use of early, alternative poly(A sites, thereby preventing the accumulation of non-functional truncated proteins. Mechanistically, associates with the phosphorylated C-terminal heptapeptide repeat domain (CTD of the largest RNA polymerase II subunit (POLR2A, and subsequently binds nascent RNA upstream of early polyadenylation sites to prevent premature mRNA transcript cleavage and polyadenylation. Independently of SCAF4, also acts as a positive regulator of transcript elongation.
Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Immunogen: Recombinant fusion protein of human SCAF8
Dilution: WB 1:500-1:1000
Calculated MW: 140kDa/147kDa
Observed MW: 170KDa
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