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Encompassing Amino Acids: 510-1290(end)
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed using the SETDB1 Chemiluminescent Assay Kit, BPS Bioscience Cat. #51056 per the manufacturer's instructions.
Description: Human SetDB1 (variant 2), also known as Histone-lysine Nmethyltransferase SETDB1, SET domain bifurcated 1, KMT1E, ERG-associated protein with SET domain (ESET), GenBank Accession No. NM_012432, a.a. 510- 1290(end) expressed in a Baculovirus Sf9 cell expression system. MW = 114 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% Glycerol and 4 mM glutathione
Genbank: NM_012432
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-GST-tags
Uniprot: Q15047
Warnings: Avoid freeze/thaw cycles. Storing diluted protein is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Dillon, S.C., et al., Genom. Biol. 2005; 6: 227.
2. Schultz, D.C. et al., Genes Dev. 2002; 16: 919-932.
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