Target Synonym: 9530036O11Rik; Dsh; Hhg1; Hx; Hxl3; M100081
Storage: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Background: Mouse Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called Hedgehog. The other twofamily members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signalingmolecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles inregulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult,including the division of adult stem cells and the development of certain cancers and other diseases.Mouse Shhis synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region. Themature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa N-terminal fragment (Shh-N), and a catalytic-processing,glycosylated, 34 kDa, 239 aa C-terminal fragment. The 20 kDa Shh-N fragment isthe core of the active hedgehog molecule. Mouse Shh-N is 99%, 98%, and 100% aa identical to human, rat andgerbil Shh-N, respectively.
Buffer: PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Immunogen: Recombinant fusion protein of mouse SHH
Dilution: WB 1:500-1:2000
Observed MW: 45KDa
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