Clone: R023
Specificity: Mouse TNF-alpha. Has cross-reactivity with Human TNF-alpha/TNFA in ELISA assay
Antibody Modification: Preservative Free Unconjugated
Presentation: 0.2 µm filtered histidine and arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH 6.0
Immunogen: Recombinant Mouse TNF-alpha / TNFA / TNFSF2 Protein
Gene: TNF Alpha
Description: TNF Alpha antibody LS-C819419 is an unconjugated rabbit monoclonal antibody to mouse TNF Alpha. Validated for Block, ELISA and Neut.
Usage: Block: In a functional ELISA which immobilized Recombinant Mouse TNF-alpha/TNFA Protein at 0.2 µg/ml (100 µL/well) in the plate, the Rabbit Anti-Mouse TNF-alpha Monoclonal Antibody can block the binding of 2 µg/ml of Recombinant Mouse TNF-alpha to Human TNFR1, the EC50 is 0.33 µg/ml;In a functional ELISA which immobilized Recombinant Mouse TNF-alpha/TNFA Protein at 0.2 µg/ml(100 µL/well) in the plate, the Rabbit Anti-Mouse;Neutralization: The neutralization anctivity of Mouse TNF-alpha Antibody measured by its ability to neutralize TNF-alpha induced cytotoxicity in the L-929 mouse fibroblast cell line. Please Note: Optimal concentrations/dilutions should be determined by the end user.
Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2
Recommended Storage: Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
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