Clone: R204
Specificity: Mouse TNFR2/TNFRSF1B. No cross-reactivity with Human TNFR2/TNFRSF1B, or Cynomolgus TNFR2/TNFRSF1B in ELISA assay
Antibody Modification: Preservative Free Unconjugated
Presentation: 0.2 µm filtered histidine and arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH 6.0
Immunogen: Recombinant Mouse TNFR2/TNFRSF1B/CD120b protein
Gene: TNFRSF1B / TNFR2
Description: TNFR2 antibody LS-C819425 is an unconjugated rabbit monoclonal antibody to mouse TNFR2 (TNFRSF1B). Validated for Block, ELISA and Neut.
Usage: Block: In a functional ELISA which immobilized Mouse TNFa at 0.4 ug/ml (100 µL/well) in the plate, the Rabbit anti-Mouse TNFR2/TNFRSF1B Monoclonal Antibody can block the binding of 0.5 ug/ml of biotinylated Mouse TNFR2/TNFRSF1B to Mouse TNFa, the EC50 is 0.2-0.7 ug/ml. Neutralization: The neutralization activity of mouse TNFRSF1B neutralizing antibody is measured by its ability to neutralize mTNFRSF1B-mediated inhibition of cytotoxicity in the L-929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 16-50 ng/mL in the presence of 25ng/mL mTNFRSF1B, 0.5ng/mL mouse TNFa, and 1ug/mL actinomycin D. Please Note: Optimal concentrations/dilutions should be determined by the end user.
Synonyms: TNFRSF1B, CD120b antigen, CD120b, p75 TNF receptor, Soluble TNFR1B variant 1, TNFBR, Tumor necrosis factor receptor, TNF-R-II, TNF-R75, TNFR2, p75TNFR, p80 TNF-alpha receptor, TBPII, TNF-R2, TNFR80, TNF-RII, TNFR-II, TNFR1B
Recommended Storage: Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Concentration: 2.64 µg/µl
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