Clone: R001
Specificity: Human RANKL. Has cross-reactivity with Cynomolgus TNFSF11 in ELISA assay. No cross-reactivity with Mouse TNFSF11 in ELISA assay
Antibody Modification: Preservative Free Unconjugated
Presentation: 0.2 µm filtered histidine and arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH 6.0
Immunogen: Recombinant Human RANKL / OPGL / TNFSF11 / CD254 protein
Gene: TNFSF11 / RANKL / TRANCE
Description: TRANCE antibody LS-C819140 is an unconjugated rabbit monoclonal antibody to human TRANCE (TNFSF11 / RANKL). Validated for Block, ELISA and Neut.
Usage: Block: In a functional ELISA which immobilized recombinant Human TNFRSF11A at 2ug/ml (100 µL/well) in the plate, the Rabbit anti-Human TNFSF11 Monoclonal Antibody can block the binding of 0.2 ug/ml of recombinant Human TNFSF11 to human TNFRSF11A, the EC50 is 0.48 ug/ml; Immobilized recombinant Human recombinant Human TNFRSF11B at 2 ug/ml in the plate, the Antibody can block the binding of 0.2 ug/ml of recombinant Human TNFSF11 to human TNFRSF11B, the EC50 is 1.02 ug/ml. Neutralization: The neutralization activity of TNFSF11 Neutralizing Antibody is Measured by its ability to neutralize TNFSF11 induced osteoclast-like cell formation in RAW 264. 7 mouse macrophages. The Neutralization titer (IC50) is typically 4-16 ng/mL in the presence of 30 ng/mL Recombinant Human RANKL/OPGL/TNFSF11/CD254. Please Note: Optimal concentrations/dilutions should be determined by the end user.
Synonyms: TNFSF11, CD254, CD254 antigen, HRANKL2, OPTB2, TRANCE, ODF, OPGL, SOdf, Osteoprotegerin ligand, RANKL
Recommended Storage: Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
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