Description: This MAb recognizes proteins of 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet-derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: VG76e
Immunogen: Human VEGF189 recombinant protein
Antibody Reactivity: VEGF
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- Endocrinology (2000) 141(3): 995-1000. (WB, functional studies)
- J Vet Diag Invest (2010) 22(1):105-108. (IHC, FFPE)
Entrez Gene ID: 7422
Expected AB Applications: Functional studies (published for clone)/IHC (FFPE) (published for clone)/WB (published for clone)
Z-Antibody Applications: Functional studies (published)/IHC, FFPE (published)/WB (published)
Antibody Application Notes: ELISA: For coating, order Ab without BSA; Immunofluorescence: 1-2 ug/mL; Optimal dilution for a specific application should be determined./Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody
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