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Encompassing Amino Acids: 207-327
Description: Recombinant human vascular endothelial growth factor (VEGF)121 expressed in CHO cells. Human VEGF exists in multiple molecular variants and are named based on the number of amino acids they contain. VEGF121 is the smallest and most soluble form of VEGF.
Endotoxin Level: Endotoxin level was found to be < 0.1 ng/g (1EU/g), using the LAL gel clot method.
Format: lyophilized protein
Formulation: Lyophilized powder
Genbank: NM_AF214570.1
Host Cell Line: CHO
Purity: >95%, as determined by SDSPAGE and HPLC.
Reconstitution: Briefly centrifuge the vial, followed by reconstitution in distilled water to a concentration of ≥0.1 mg/ml. This solution can then be further diluted into other buffers containing carrier protein (0.1% BSA).
Storage Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at 2 - 8° C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Uniprot: P15692
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Reproduction. 2009; 138: 667-677.
2. Ann. Onc. 2009; 20: 1639-1646.
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