Clone: R010
Specificity: Human VEGFA. Has cross-reactivity with mouse VEGF164 and rat VEGF164 in ELISA assay
Antibody Modification: Preservative Free Unconjugated
Presentation: 0.2 µm filtered solution in PBS
Immunogen: Recombinant Human VEGF165/VEGFA Protein
Gene: VEGFA / VEGF
Description: VEGF antibody LS-C819200 is an unconjugated rabbit monoclonal antibody to human VEGF (VEGFA). Validated for Block, ELISA and Neut.
Usage: Block: In a functional ELISA which immobilized recombinant Human VEGF165 at 1 ug/ml (100 µL/well) in the plate, the Rabbit anti-Human VEGF165 Monoclonal Antibody can block the binding of 5 ug/ml of biotinylated recombinant Human VEGFR2 to human VEGF165, the EC50 is 1. 51 ug/ml. Neutralization: The neutralization activity of VEGF Neutralizing Antibody is measured by its ability to neutralize VEGF165 induced proliferation in HUVEC human umbilical vein endothelial cells. The VEGF Neutralization titer (IC50) is typically 5. 35-21. 4 ng/mL in the presence of 10 ng/mL Recombinant Human VEGF165. Please Note: Optimal concentrations/dilutions should be determined by the end user.
Synonyms: VEGFA, VPF, Vascular permeability factor, VEGF, VEGF-A, MVCD1
Recommended Storage: Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Concentration: 1.85 mg/ml
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