Application Note: IHC-P: 3-6μg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Positive Control: The peptide used to generate this antibody is available for purchase (GTX89612-PEP).
Specificity/Sensitivity: This antibody is expected to recognise isoform d (NP_597673.1) only.
Form: Liquid
Buffer (with preservative): TBS, 0.5% BSA, 0.02% Sodium azide.
Concentration: 0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Background: This gene is a member of the XAGE subfamily, which belongs to the GAGE family. The GAGE genes are expressed in a variety of tumors and in some fetal and reproductive tissues. This gene is strongly expressed in Ewing's sarcoma, alveolar rhabdomyosarcoma and normal testis. The protein encoded by this gene contains a nuclear localization signal and shares a sequence similarity with other GAGE/PAGE proteins. Because of the expression pattern and the sequence similarity, this protein also belongs to a family of CT (cancer-testis) antigens. Alternative splicing of this gene, in addition to alternative transcription start sites, results in multiple transcript variants. [provided by RefSeq, Jan 2010]
Antigen Species: Human
Immunogen: Peptide with sequence CGFGFRRQGEDNT, from the C Terminus of the protein sequence according to NP_597673.1.
Purification: Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation: Unconjugated