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Encompassing Amino Acids: 208-505
Application: Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.
Background: FRK (fyn-related kinase), also called PTK5 (protein tyrosine kinase 5) is a nuclear tyrosine kinase and member of the Src sub-family. Restricted expression of FRK is detected in a broad range of cell lines with highest levels in epithelial cells. Increased expression of FRK has been shown in breast and renal cell carcinoma cell lines. It was initially thought to be a tumor suppressor, but more recently it has also been shown to participate in tumor progression, such as in the case of pancreatic cancer and leukemia. Its role may therefore be tissue specific. In addition, it plays a role in antiviral immune responses by interacting with TBK1 (TANK-binding kinase 1). Overexpression of FRK in beta-cells from the pancreas increases the susceptibility of these cells to beta-cell-toxic events (hallmark of Type I diabetes). The development of therapeutic strategies to target FRK can be a promising area of research towards several clinical applications.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The Chemi-Verse™ FRK Kinase Assay Kit is designed to measure FRK (Fyn-related kinase) tyrosine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant FRK kinase (amino acids 208-505), kinase substrate, ATP and kinase assay buffer for 100 enzyme reactions.Need us to run inhibitor screens or profile your compounds ? Check out our Kinase Screening Services.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The assay kit comes in a convenient 96-well format, with enough purified recombinant FRK kinase, kinase substrate, ATP and kinase assay buffer for 100 enzyme reactions.
Target: FRK
Uniprot: P42685
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Zhang M., et al., 2025 Front. Microl. 16: https://doi.org/10.3389/fmicb.2025.1525648.Ogunbolude Y., et al., 2017 Oncotarget 8(68): 113034-113065.
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