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Application: Study enzyme kinetics, and screen small molecular inhibitors of histone demethylases for HTS applications in drug discovery.
Description: The 4x HDM Incomplete Assay Buffer does not contain -ketoglutarate and provides a more accurate background measure than using a no-enzyme control. It has been optimized for use with the following BPS Bioscience histone demethylase assay kits: / FBXL10 (KDM2B) Homogeneous Assay Kit, BPS Cat. #50610 / FBXL11 (KDM2A) Homogeneous Assay Kit, BPS Cat. #50611 / JARID1A (KDM5A) Homogeneous Assay Kit, BPS Cat. #50510 / JARID1B (KDM5B) Homogeneous Assay Kit, BPS Cat. #50512 / JARID1C (KDM5C) Homogeneous Assay Kit, BPS Cat. #50511 / JHDM1D (KDM7A) Homogeneous Assay Kit, BPS Cat. #50420 / JMJD1A (KDM3A) Homogeneous Assay Kit, BPS Cat. #50412 / JMJD2A (KDM3A) Homogeneous Assay Kit, BPS Cat. #50413 / JMJD2B (KDM4B) Homogeneous Assay Kit, BPS Cat. #50414 / JMJD2C (KDM4C) Homogeneous Assay Kit, BPS Cat. #50415 / JMJD2E (KDM4DL) Homogeneous Assay Kit, BPS Cat. #50417 / JMJD3 (KDM6B) Homogeneous Assay Kit, BPS Cat. #50416 / PHF8 (KDM7B) Homogeneous Assay Kit, BPS Cat. #50515 / UTX (KDM6A) Homogeneous Assay Kit, BPS Cat. #50516
Format: Aqueous buffer solution
Storage Stability: Stable for at least 1 year when stored as directed.
Supplied As: HEPES-based buffer containing reducing agent
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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