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Applications: Useful as a reference antibody for screening TIGIT neutralizing antibodies in cell based assays.
Assay Conditions: Assays done according to protocols from TIGIT:CD155 homogenous assays (72029). CD155-his was mixed with various concentrations of the anti-TIGIT mAbs (0-100 nM) and TIGIT-biotin for 1h at RT. Then Strep donor (1:125) and Ni acceptor beads (1:250) were added for 1h. Plate read on the alphaLISA machine
Description: Human Anti-TIGIT neutralizing antibody recognizing the TIGIT binding region on immune cells.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Purification: Protein A affinity chromatography from HEK293 supernatants.
Storage Stability: Stable for at least 12 months at -80°C. Store diluted antibody with carrier protein (BSA 0.1 - 0.5%) at 4°C, stable for 2 weeks.
Uniprot: Q495A1
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1.Yu, X., et al., Nat. Immunol. 2009; 10(1): 48-57.2.Stanietsky, N., et al., Proc. Natl. Acad. Sci. (USA). 2009; 106(42): 17858-17863.
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