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Encompassing Amino Acids: CDK9: 2- 372(end); CyclinT1: 2-726(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done in 50 μL assay buffer containing 25 mM Tris, pH 7.4, 5 mM MgCl2 and 100 μM AMP for 15 min at 37°C. Phosphate standard solution was run side-by-side for product quantification.
Description: Human cyclin-dependent kinase 9 (CDK9), also known as C-2K and cell division protein kinase 9, complexed with human cyclin T1 (CCNT1, CycT1), GenBank Accession Nos. NM_001261 (CDK9) and NM_001240 (CyclinT1), a.a. 2- 372(end) (CDK9) and a.a. 2-726(end) (CyclinT1) each with N-terminal GST-tags, co-expressed in a baculovirus-infected Sf9 cell expression system. MW = 69 kDa (CDK9) and 107 kDa (CyclinT1).
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04%Tween-20, 3 mM DTT, 20% glycerol, and1.6 mM glutathione
Genbank: CDK9: NM_001261; CyclinT1: NM_001240
Host Cell Line: Sf9 cells
Purity: ≥60%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: CDK9: P50750; CyclinT1: O60563
Warnings: Avoid freeze/thaw cycles. Storing diluted protein is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Yang, Z., et al., Nat. 2001; 414: 317-322.2. Baumli, S., et al., EMBO J. 2008; 27(13): 1907-1918.
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