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Applications: Useful as CD20-expressing target cells in co-culture assays with CD20-CAR-T cells for CD20-specific cell killing assays.
Background: CD20 (MS4A1) is a glycosylated phosphoprotein expressed on the cell surface of B cells. Although the functional significance of CD20 is not clear, and CD20 has no known ligands, CD20 has been shown to regulate intracellular calcium levels. CD20 is a highly attractive target antigen for immunotherapy because it is expressed on more than 90% of patients with B-cell lymphoma. First approved in 1997, Rituximab (Rituxan) is a chimeric monoclonal antibody targeting CD20 and has been classified by the World Health Organization as an “Essential Medicine”.Since then, additional monoclonal antibodies against CD20 have been approved or are being tested in clinical trials for the treatment of multiple sclerosis (MS), chronic lymphocytic leukemia (CLL), follicular lymphoma, diffuse large B cell lymphoma (DLBCL), rheumatoid arthritis, non-Hodgkin’s lymphoma, systemic lupus erythematosus, and myalgic encephalomyelitis (chronic fatigue syndrome). Additionally, more recently, anti-CD20-CD19 bispecific CAR-T cells have been developed to address concerns over potential relapse.
Description: Recombinant clonal stable CHO cell line constitutively expressing full length human CD20 protein, also known as MS4A1 (Genbank #NM_152866). Surface expression of CD20 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of CD20 expression (High, Medium) to mimic different stages of cancer target cells with various CD20 expression levels.
Genbank: NM_152866
Host Cell Line: CHO
Mycoplasma Testing: This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.
Storage Stability: Store in liquid nitrogen immediately upon receipt.
Supplied As: Each vial contains ~ 2 x 10^6 cells in 1 ml of 10% DMSO in FBS.
Uniprot: P11836
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
References: 1. Kosmas, C, et al. Anti-CD20-based therapy of B cell lymphoma: state of the art. Leukemia. 2002; 16: 2004-20152. Cragg, MS, et al. The biology of CD20 and its potential as a target for mAb therapy. Curr Dir Autoimmun. 2005; 8: 140-1743. Martyniszyn, A, et al. CD20-CD19 bispecific CAR T cells for the treatment of B cell malignancies. Hum Gene Ther. 2017 Dec; 28(12): 1147-11574. Lee, SY, et al. Preclinical optimization of a CD20-specific chimeric antigen receptor vector and culture conditions. J Immunother. 2018 Jan; 41(1): 19-31
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