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Applications: Screen for TRβ agonists.
Background: Thyroid hormones play an important role in growth, development, and metabolism. These are mediated by two different thyroid hormone receptor (TR) isoforms, TRα and TRβ. Thyroid receptors are transcriptional factors that control various genes by interacting with specific co-activators, co-repressors and DNA sequences. In humans, TRα is the predominant form of the thyroid receptor in the heart, brain, and bone while TRβ is mainly expressed in the liver, kidney and brain. Interestingly, it has been shown that the patients with non-alcoholic steatohepatitis (NASH) display lower TRβ expression in the liver, and more importantly, TRβ agonist treatment decreased liver steatosis and circulating lipids as well as showed metabolic benefits in preclinical models of diabetes and obesity.
Description: The TRβ-GAL4 Luciferase Reporter HEK293 Cell Line is a HEK293 cell line expressing firefly luciferase under the control of the GAL4 upstream activation sequence (UAS) with constitutive expression of human thyroid receptor β ligand binding domain (TRβ LBD, amino acids 173-461) fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD). This system allows specific detection of thyroid hormone-induced activation of the thyroid receptor β with low cross-reactivity from other nuclear receptors. This cell line has been validated by stimulation with triiodothyronine (T-3).
Host Cell Line: HEK293, Human Embryonic Kidney, epithelial-like cells, adherent.
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.
Supplied As: Each vial contains ?1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
References: Paguio A, et al., 2010 Curr Chem Genomics. 4: 43-49.
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