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Applications: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.
Background: Aurora Kinase B, also known as serine/threonine-protein kinase 5, is part of the Aurora subfamily. Aurora Kinase B is a CPC (chromosomal passenger complex) protein. It plays a role in microtubule organization during mitosis and meiosis, forming a complex with Survinin, Borealin and INCENP (inner centromere protein) and binding to EB1 (end-binding protein 1). It has a pattern of expression and subcellular localization that varies with the cell cycle stage, in agreement with its role as cell cycle regulator. It has oncogenic activity, being able to lead to the development of cells with abnormal number of chromosomes. Aurora Kinase B also promotes cancer by decreasing expression of p21, hyperactivating CDK1 (cyclin-dependent kinase 1) and regulating class IIa HDACs (histone deacetylase). Interestingly, the use of Aurora inhibitors can result in an increase in the number of chromosome alterations in cancer cells, resulting in cell death, and can also decrease expression of cyclin B1 and D1 and elevate caspase 3 levels in lymphoma cells. Further studies will elucidate the exact mode of action of Aurora Kinase B in cancer development and progression and open new therapeutic avenues.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The Chemi-Verse™ Aurora Kinase B Assay Kit is a luminescence assay designed to measure Aurora Kinase B kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant Aurora Kinase B, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed
Uniprot: Q96GD4
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Tang A., et al., 2017 Oncotarget 8(14):23937-23954.
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