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Encompassing Amino Acids: 2-1239 (end)
Applications: Useful for DNA synthesis and DNA repair, as an antigen for Western blotting, and for inhibitor screening.
Background: POLG is the catalytic subunit of the mitochondrial DNA polymerase. The catalytic subunit contains three enzymatic activities, a DNA polymerase activity, a 3'-5' exonuclease activity that proofreads misincorporated nucleotides, and a 5'-dRP lyase activity required for base excision repair. Mutations in the POLG gene are associated with several mitochondrial diseases, including Alpers' disease, ataxia-neuropathy disorders, and dominant and recessive forms of progressive external ophthalmoplegia.
Description: Human DNA polymerase γ (gamma), also known as Pol gamma and POLG (GenBank Accession No. NM_002693), a.a. 2-1239 (end), with N-terminal His-tag and N-terminal FLAG-tag, MW=141 kDa, expressed in a baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 µg/ml FLAG peptide, 0.04% Tween-20, and 20% glycerol.
Genbank: NM_002693
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-FLAG-tag
Uniprot: P54098
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Ropp, P.A., and Copeland, W.C., Genomics 1996;36(3):449-458.
2. Lestienne, P., Biochem. Biophys. Res. Comm. 1987;146(3): 1146-1153.Application Reference(s):La, J., et al. Proc Natl Acad Sci USA. 2015 Jun 2;112(22):6979-84. " Identification of mechanistically distinct inhibitors of HIV-1 reverse transcriptase through fragment screening"
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