- R-Biopharm RT PCR identification of MRSA
- Staphylococcus aureus
- Methicillin-Resistant S. aureus (MRSA)
- Test principle of RIDA® GENE MRSA
- Your advantages with RIDA® GENE MRSA
Our partner R-Biopharm has a reliable RT PCR kit for the detection of methicillin resistant Staphylococcus aureus (MRSA) and methicillin sensitive Staphylococcus aureus (MSSA).
RIDA® GENE MRSA - rapid identification of methicillin resistant S.aureus (MRSA)
The RIDA® GENE MRSA Kit is a real-time multiplex PCR for the direct qualitative detection and differentiation of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA) or methicillin-resistant coagulase-negative staphylococci from human nasal and nasopharyngeal swabs, culture samples, wound and groin swabs, as well as armpit and perineal swabs.
S.aureus is a symbiotic as well as pathogenic organism, which is often found in the nose, but also in other parts of the body. In 20% of individuals, colonization is persistent, with 30% being periodic.
Due to colonization, the possibilities of transmission in both public life and medical facilities are very common.
S. aureus is the second most common germ that occurs in positive blood cultures. Coagulase-negative S.aureus (KNS) is the most common cause of circulatory infection (about 42%) and S.aureus (about 16.5%) the second leading cause of infection. Some strains are resistant to β-lactams, e.g. Methicillin, and therefore therapy should be optimized for MRSA and MSSA infections.
MRSA is a known resistant pathogen that is very common in public life as well as in medical settings. The resistance is due to the presence and expression of the mecA gene, which expresses the unique penicillin-binding protein (PBP2a) with low affinity for methicillin and other beta-lactams. MRSA infections can increase hospital costs and prolong hospital stay for patients.
RIDA®GENE MRSA is a multiplex real-time PCR for the direct qualitative detection and differentiation of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA) or coagulase-negative methicillin-resistant staphylococci. After DNA isolation (if present), the mecA/mecC gene, SCCmec/orfX junction (types I, II, III, IV, V, VI, VII, IX, X, XI) and the SA442 gene, specific to MRSA, are amplified.
The amplified target sequences are detected with hydrolysis probes labeled with the quencher at one end and a reporter fluorescent dye (fluorophore) at the other end. In the presence of a target sequence, the probes hybridize with the amplicons. During the extension, the Taq polymerase separates the reporter from the quencher. The reporter emits a fluorescence signal that is detected by the optical unit of a real-time PCR device.
✔ direct detection of mecA / mecC gene, SCCmec / orfX junction (types I, II, III, IV, V, VI, VII, IX, X, XI) and the SA442 gene
✔ Real Time Multiplex PCR
✔ runs on all common cyclers
✔ fast and accurate detection of MRSA and MSSA
✔ very sensitive method (detection limit of ≥ 10 DNA copies / reaction)
✔ DNA isolation from smear or culture samples