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Encompassing Amino Acids: 25-167
Applications: Useful for studying protein binding and screening small molecular inhibitors
Assay Conditions: 10 nM PD-L1, Biotinlabeled(BPS Cat. #71105) was mixed withvarying amounts of PD-1, FLAG-tag (BPSCat. #71198) in PD-1 Assay Buffer for 1 hrat RT. FLAG-acceptor beads (left) or Nickel-acceptorbeads (right) were added andincubated for 30 min at RT followed bystreptavidin-conjugated donor beads for 30min at RT. Alpha counts were read.
Description: Human secreted programmed cell death 1 (PD-1), also known as PDCD1, SLEB2, CD279 and HPD-L, GenBank Accession No. NM_005018, a.a. 25-167, with C-terminal FLAG-Avi-His-tags, MW=20 kDa, expressed in a HEK293 cell expression system. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate pH 7.4,110 mM NaCl, 2.2 mM KCl, and 20%glycerol
Genbank: NM_005018
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal FLAG-Avi- His-tag
Uniprot: Q15116
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Shinohara, T., et al., Genomics. 1994 Oct;23(3):704-6.
2. James, E.S., et al., Genes Immun. 2005 Aug;6(5):430-7.
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