Immunohistochemistry

Immunohistochemistry

Immunohistochemistry (IHC) is an immune-based staining technique enabling antibody detection of specific antigens in tissue samples.

There are three main methods used in IHC: direct, indirect and enzyme „sandwich“ methods.

Direct IHC uses directly labelled (conjugated) primary antibodies while indirect IHC-detection uses unlabeled primary and labelled secondary antibodies. Both primary and secondary antibodies might be conjugated directly either to enzymes most commonly to horseradish peroxidase (HRP) or alkaline phosphatase (AP) or fluorophors. The sandwich method uses specific streptavidin or avidin binding reaction with biotin, which supports signal amplification, and might be preferable when detecting weakly-expressed antigens. Samples using enzymatic detection also need additionally enzyme substrates, which are enzymatically converted into stable chromogen products. These stable products can then be visualized under a light microscope.

We offer a broad range of primary and secondary antibodies, both unconjugated and conjugated to biotin, HRP, AP and various fluorophores. Available in our Webshop are also detection systems (avidin-biotin based, polymersystems), enzyme substrates, mounting media and buffers and solution for your successful immunohistochemical experiments.

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  1. Biotin Western Blot Anti-Human IgG Antibody Maxtag™
    Biotin Western Blot Anti-Human IgG Antibody Maxtag™
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    Sku ROCKIB001
    Manufact. SkuKIB001
    Packaging Unit1 mL
  2. Biotin Western Blot Anti-Mouse IgG Antibody Maxtag™
    Biotin Western Blot Anti-Mouse IgG Antibody Maxtag™
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    Sku ROCKIB002
    Manufact. SkuKIB002
    Packaging Unit1 mL
  3. Biotin Western Blot Anti-Rabbit IgG Antibody Maxtag™
    Biotin Western Blot Anti-Rabbit IgG Antibody Maxtag™
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    Sku ROCKIB003
    Manufact. SkuKIB003
    Packaging Unit1 mL
  4. Biotin Western Blot Anti-Goat IgG Antibody Maxtag™
    Biotin Western Blot Anti-Goat IgG Antibody Maxtag™
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    Sku ROCKIB004
    Manufact. SkuKIB004
    Packaging Unit1 mL
  5. Streptavidin-Peroxidase Conjugate for Western Blot MaxTag™
    Streptavidin-Peroxidase Conjugate for Western Blot MaxTag™
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    Sku ROCKID001
    Manufact. SkuKID001
    Packaging Unit1 mL
  6. Blocking Buffer for Fluorescent Western Blotting 3-PACK (3 x 500 ml)
    Blocking Buffer for Fluorescent Western Blotting 3-PACK (3 x 500 ml)
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    Sku ROCMB-070-003
    Manufact. SkuMB-070-003
    Packaging Unit3 x 500 mL
  7. Blocking Buffer (2X) for Fluorescent Western Blotting
    Blocking Buffer (2X) for Fluorescent Western Blotting
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    Sku ROCMB-070-0050
    Manufact. SkuMB-070-0050
    Packaging Unit50 mL
  8. Blocking Buffer for Fluorescent Western Blotting 10-PACK
    Blocking Buffer for Fluorescent Western Blotting 10-PACK
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    Sku ROCMB-070-010
    Manufact. SkuMB-070-010
    Packaging Unit10 x 500 mL
  9. Blocking Buffer for Fluorescent Western Blotting (Thimerosal Free) 10-Pack
    Blocking Buffer for Fluorescent Western Blotting (Thimerosal Free) 10-Pack
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    Sku ROCMB-070-010TF
    Manufact. SkuMB-070-010TF
    Packaging Unit10 x 500 mL
  10. Blocking Buffer (10X) for Fluorescent Western Blotting (Thimerosal Free)
    Blocking Buffer (10X) for Fluorescent Western Blotting (Thimerosal Free)
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    Sku ROCMB-070-050TF
    Manufact. SkuMB-070-050TF
    Packaging Unit50 mL
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