Immunohistochemistry

Immunohistochemistry

Immunohistochemistry (IHC) is an immune-based staining technique enabling antibody detection of specific antigens in tissue samples.

There are three main methods used in IHC: direct, indirect and enzyme „sandwich“ methods.

Direct IHC uses directly labelled (conjugated) primary antibodies while indirect IHC-detection uses unlabeled primary and labelled secondary antibodies. Both primary and secondary antibodies might be conjugated directly either to enzymes most commonly to horseradish peroxidase (HRP) or alkaline phosphatase (AP) or fluorophors. The sandwich method uses specific streptavidin or avidin binding reaction with biotin, which supports signal amplification, and might be preferable when detecting weakly-expressed antigens. Samples using enzymatic detection also need additionally enzyme substrates, which are enzymatically converted into stable chromogen products. These stable products can then be visualized under a light microscope.

We offer a broad range of primary and secondary antibodies, both unconjugated and conjugated to biotin, HRP, AP and various fluorophores. Available in our Webshop are also detection systems (avidin-biotin based, polymersystems), enzyme substrates, mounting media and buffers and solution for your successful immunohistochemical experiments.

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  1. TMB Stop Buffer, CE-IVD
    TMB Stop Buffer, CE-IVD
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    Sku SCYTSB-20000
    Manufact. SkuTSB-20000
    Packaging Unit20 L
  2. Trubond 380 Microscope Slides, CE-IVD
    Trubond 380 Microscope Slides, CE-IVD
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    Sku SCYTRU380
    Manufact. SkuTRU380
    Packaging Unit100 Slides
  3. TMB Precipitating (High Sensitivity), CE-IVD
    TMB Precipitating (High Sensitivity), CE-IVD
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    Sku SCYTM5010
    Manufact. SkuTM5010
    Packaging Unit10 L
  4. TMB Precipitating (Standard Sensitivity), CE-IVD
    TMB Precipitating (Standard Sensitivity), CE-IVD
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    Sku SCYTM3010
    Manufact. SkuTM3010
    Packaging Unit10 L
  5. TC Indicator TM0125, CE-IVD
    TC Indicator TM0125, CE-IVD
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    Sku SCYTHF500
    Manufact. SkuTHF500
    Packaging Unit500 ml
  6. Tris-EDTA HIER Solution (10x) pH 9.0, CE-IVD
    Tris-EDTA HIER Solution (10x) pH 9.0, CE-IVD
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    Sku SCYTES-20000
    Manufact. SkuTES-20000
    Packaging Unit20 L
  7. Silver Nitrate Solution (10%), CE-IVD
    Silver Nitrate Solution (10%), CE-IVD
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    Sku SCYSNX999
    Manufact. SkuSNX999
    Packaging Unit1000 ml
  8. Silver Nitrate Solution (10%), CE-IVD
    Silver Nitrate Solution (10%), CE-IVD
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    Sku SCYSNX010
    Manufact. SkuSNX010
    Packaging Unit10 ml
  9. Schmorl's Stain Kit, CE-IVD
    Schmorl's Stain Kit, CE-IVD
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    Sku SCYSML-2
    Manufact. SkuSML-2
    Packaging Unit1 Kit
  10. Schmorl's Stain Kit, CE-IVD
    Schmorl's Stain Kit, CE-IVD
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    Sku SCYSML-1
    Manufact. SkuSML-1
    Packaging Unit1 Kit
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