Immunohistochemistry

Immunohistochemistry

Immunohistochemistry (IHC) is an immune-based staining technique enabling antibody detection of specific antigens in tissue samples.

There are three main methods used in IHC: direct, indirect and enzyme „sandwich“ methods.

Direct IHC uses directly labelled (conjugated) primary antibodies while indirect IHC-detection uses unlabeled primary and labelled secondary antibodies. Both primary and secondary antibodies might be conjugated directly either to enzymes most commonly to horseradish peroxidase (HRP) or alkaline phosphatase (AP) or fluorophors. The sandwich method uses specific streptavidin or avidin binding reaction with biotin, which supports signal amplification, and might be preferable when detecting weakly-expressed antigens. Samples using enzymatic detection also need additionally enzyme substrates, which are enzymatically converted into stable chromogen products. These stable products can then be visualized under a light microscope.

We offer a broad range of primary and secondary antibodies, both unconjugated and conjugated to biotin, HRP, AP and various fluorophores. Available in our Webshop are also detection systems (avidin-biotin based, polymersystems), enzyme substrates, mounting media and buffers and solution for your successful immunohistochemical experiments.

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  1. T98G Cell Lysate
    T98G Cell Lysate
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    Sku SACSC-2294
    Manufact. Skusc-2294
    Packaging Unit500 µg/200 µl
  2. T-47D Cell Lysate
    T-47D Cell Lysate
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    Sku SACSC-2293
    Manufact. Skusc-2293
    Packaging Unit500 µg/200 µl
  3. T24 Cell Lysate
    T24 Cell Lysate
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    Sku SACSC-2292
    Manufact. Skusc-2292
    Packaging Unit500 µg/200 µl
  4. SK-MEL-28 + IFN-γ Cell Lysate
    SK-MEL-28 + IFN-γ Cell Lysate
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    Sku SACSC-2291
    Manufact. Skusc-2291
    Packaging Unit500 µg/200 µl
  5. SK-MEL-28 + IFN-α Cell Lysate
    SK-MEL-28 + IFN-α Cell Lysate
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    Sku SACSC-2290
    Manufact. Skusc-2290
    Packaging Unit500 µg/200 µl
  6. SK-N-SH + nocodazole Cell Lysate
    SK-N-SH + nocodazole Cell Lysate
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    Sku SACSC-2289
    Manufact. Skusc-2289
    Packaging Unit500 µg/200 µl
  7. SJRH30 Cell Lysate
    SJRH30 Cell Lysate
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    Sku SACSC-2287
    Manufact. Skusc-2287
    Packaging Unit500 µg/200 µl
  8. MIA PaCa-2 Cell Lysate
    MIA PaCa-2 Cell Lysate
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    Sku SACSC-2285
    Manufact. Skusc-2285
    Packaging Unit500 µg/200 µl
  9. MES-SA/Dx5 Cell Lysate
    MES-SA/Dx5 Cell Lysate
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    Sku SACSC-2284
    Manufact. Skusc-2284
    Packaging Unit500 µg/200 µl
  10. MEG-01 Cell Lysate
    MEG-01 Cell Lysate
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    Sku SACSC-2283
    Manufact. Skusc-2283
    Packaging Unit500 µg/200 µl
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