Immunohistochemistry

Immunohistochemistry

Immunohistochemistry (IHC) is an immune-based staining technique enabling antibody detection of specific antigens in tissue samples.

There are three main methods used in IHC: direct, indirect and enzyme „sandwich“ methods.

Direct IHC uses directly labelled (conjugated) primary antibodies while indirect IHC-detection uses unlabeled primary and labelled secondary antibodies. Both primary and secondary antibodies might be conjugated directly either to enzymes most commonly to horseradish peroxidase (HRP) or alkaline phosphatase (AP) or fluorophors. The sandwich method uses specific streptavidin or avidin binding reaction with biotin, which supports signal amplification, and might be preferable when detecting weakly-expressed antigens. Samples using enzymatic detection also need additionally enzyme substrates, which are enzymatically converted into stable chromogen products. These stable products can then be visualized under a light microscope.

We offer a broad range of primary and secondary antibodies, both unconjugated and conjugated to biotin, HRP, AP and various fluorophores. Available in our Webshop are also detection systems (avidin-biotin based, polymersystems), enzyme substrates, mounting media and buffers and solution for your successful immunohistochemical experiments.

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  1. TMB ELISA Peroxidase Substrate
    TMB ELISA Peroxidase Substrate
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    Sku ROCTMBE-1000
    Manufact. SkuTMBE-1000
    Packaging Unit1 L
  2. TMB ELISA Peroxidase Substrate (BULK ORDER)
    TMB ELISA Peroxidase Substrate (BULK ORDER)
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    Sku ROCTMBE-20L
    Manufact. SkuTMBE-20L
    Packaging Unit10 x 2 L
  3. TMB ELISA Peroxidas Substrate (BULK ORDER)
    TMB ELISA Peroxidas Substrate (BULK ORDER)
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    Sku ROCTMBE-8000
    Manufact. SkuTMBE-8000
    Packaging Unit8 x 1 L
  4. TMB Prestained Red ELISA Peroxidase Substrate
    TMB Prestained Red ELISA Peroxidase Substrate
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    Sku ROCTMBE-RED-0100
    Manufact. SkuTMBE-RED-0100
    Packaging Unit100 mL
  5. TMB Prestained Red ELISA Peroxidase Substrate
    TMB Prestained Red ELISA Peroxidase Substrate
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    Sku ROCTMBE-RED-1000
    Manufact. SkuTMBE-RED-1000
    Packaging Unit1000 mL
  6. TMB Membrane Peroxidase Substrate - Ready-To-Use
    TMB Membrane Peroxidase Substrate - Ready-To-Use
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    Sku ROCTMBM-100
    Manufact. SkuTMBM-100
    Packaging Unit100 mL
  7. TMB Membrane Peroxidase Substrate - Ready-To-Use
    TMB Membrane Peroxidase Substrate - Ready-To-Use
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    Sku ROCTMBM-1000
    Manufact. SkuTMBM-1000
    Packaging Unit1 L
  8. TMB Membrane Peroxidase Substrate - Ready-To-Use
    TMB Membrane Peroxidase Substrate - Ready-To-Use
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    Sku ROCTMBM-20L
    Manufact. SkuTMBM-20L
    Packaging Unit10 x 2 L
  9. TMB Membrane Peroxidase Substrate - Ready-To-Use
    TMB Membrane Peroxidase Substrate - Ready-To-Use
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    Sku ROCTMBM-8000
    Manufact. SkuTMBM-8000
    Packaging Unit8 x 1 L
  10. UniGlow™ - One Component Chemiluminescent Substrate
    UniGlow™ - One Component Chemiluminescent Substrate
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    Sku ROCUniGlow-0020
    Manufact. SkuUniGlow-0020
    Packaging Unit20 mL
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33133 items

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