Immunohistochemistry

Immunohistochemistry

Immunohistochemistry (IHC) is an immune-based staining technique enabling antibody detection of specific antigens in tissue samples.

There are three main methods used in IHC: direct, indirect and enzyme „sandwich“ methods.

Direct IHC uses directly labelled (conjugated) primary antibodies while indirect IHC-detection uses unlabeled primary and labelled secondary antibodies. Both primary and secondary antibodies might be conjugated directly either to enzymes most commonly to horseradish peroxidase (HRP) or alkaline phosphatase (AP) or fluorophors. The sandwich method uses specific streptavidin or avidin binding reaction with biotin, which supports signal amplification, and might be preferable when detecting weakly-expressed antigens. Samples using enzymatic detection also need additionally enzyme substrates, which are enzymatically converted into stable chromogen products. These stable products can then be visualized under a light microscope.

We offer a broad range of primary and secondary antibodies, both unconjugated and conjugated to biotin, HRP, AP and various fluorophores. Available in our Webshop are also detection systems (avidin-biotin based, polymersystems), enzyme substrates, mounting media and buffers and solution for your successful immunohistochemical experiments.

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  1. FRP-3 (h2): 293T Lysate
    FRP-3 (h2): 293T Lysate
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    Sku SACSC-176294
    Manufact. Skusc-176294
    Packaging Unit100 µg/200 µl
  2. β ig-h3 (h): 293T Lysate
    β ig-h3 (h): 293T Lysate
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    Sku SACSC-176290
    Manufact. Skusc-176290
    Packaging Unit100 µg/200 µl
  3. Synaptogyrin-3 (h): 293T Lysate
    Synaptogyrin-3 (h): 293T Lysate
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    Sku SACSC-176288
    Manufact. Skusc-176288
    Packaging Unit100 µg/200 µl
  4. galectin-3 (h2): 293T Lysate
    galectin-3 (h2): 293T Lysate
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    Sku SACSC-176286
    Manufact. Skusc-176286
    Packaging Unit100 µg/200 µl
  5. glypican-3 (h): 293T Lysate
    glypican-3 (h): 293T Lysate
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    Sku SACSC-176285
    Manufact. Skusc-176285
    Packaging Unit100 µg/200 µl
  6. AP-3μ (h2): 293T Lysate
    AP-3μ (h2): 293T Lysate
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    Sku SACSC-176284
    Manufact. Skusc-176284
    Packaging Unit100 µg/200 µl
  7. mGluR-3 (h): 293T Lysate
    mGluR-3 (h): 293T Lysate
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    Sku SACSC-176280
    Manufact. Skusc-176280
    Packaging Unit100 µg/200 µl
  8. claudin-3 (h): 293T Lysate
    claudin-3 (h): 293T Lysate
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    Sku SACSC-176276
    Manufact. Skusc-176276
    Packaging Unit100 µg/200 µl
  9. GFRα-3 (h2): 293T Lysate
    GFRα-3 (h2): 293T Lysate
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    Sku SACSC-176275
    Manufact. Skusc-176275
    Packaging Unit100 µg/200 µl
  10. AP-3σ (h): 293T Lysate
    AP-3σ (h): 293T Lysate
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    Sku SACSC-176272
    Manufact. Skusc-176272
    Packaging Unit100 µg/200 µl
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