Immunohistochemistry

Immunohistochemistry

Immunohistochemistry (IHC) is an immune-based staining technique enabling antibody detection of specific antigens in tissue samples.

There are three main methods used in IHC: direct, indirect and enzyme „sandwich“ methods.

Direct IHC uses directly labelled (conjugated) primary antibodies while indirect IHC-detection uses unlabeled primary and labelled secondary antibodies. Both primary and secondary antibodies might be conjugated directly either to enzymes most commonly to horseradish peroxidase (HRP) or alkaline phosphatase (AP) or fluorophors. The sandwich method uses specific streptavidin or avidin binding reaction with biotin, which supports signal amplification, and might be preferable when detecting weakly-expressed antigens. Samples using enzymatic detection also need additionally enzyme substrates, which are enzymatically converted into stable chromogen products. These stable products can then be visualized under a light microscope.

We offer a broad range of primary and secondary antibodies, both unconjugated and conjugated to biotin, HRP, AP and various fluorophores. Available in our Webshop are also detection systems (avidin-biotin based, polymersystems), enzyme substrates, mounting media and buffers and solution for your successful immunohistochemical experiments.

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  1. Monkey Brain (Normal) tissue lysate, Membrane Fraction
    Monkey Brain (Normal) tissue lysate, Membrane Fraction
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    Sku GTX25069-100
    Manufact. SkuGTX25069-100
    Packaging Unit100 μg
  2. Monkey Brain (Normal) tissue lysate, Membrane Fraction
    Monkey Brain (Normal) tissue lysate, Membrane Fraction
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    Sku GTX25068-100
    Manufact. SkuGTX25068-100
    Packaging Unit100 μg
  3. Monkey Heart (Normal) tissue lysate, Membrane Fraction
    Monkey Heart (Normal) tissue lysate, Membrane Fraction
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    Sku GTX24519-100
    Manufact. SkuGTX24519-100
    Packaging Unit100 μg
  4. Monkey Tissue Array - Normal Tissues
    Monkey Tissue Array - Normal Tissues
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    Sku GTX21345-5
    Manufact. SkuGTX21345-5
    Packaging Unit5 slide
  5. Multiple Species Tissue Array I - Normal Tissues
    Multiple Species Tissue Array I - Normal Tissues
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    Sku GTX21334-5
    Manufact. SkuGTX21334-5
    Packaging Unit5 slide
  6. Multiple Species Tissue Array II - Normal Tissues
    Multiple Species Tissue Array II - Normal Tissues
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    Sku GTX21333-5
    Manufact. SkuGTX21333-5
    Packaging Unit5 slide
  7. Mouse Bone Marrow-derived Dendritic Cells (BMDC) Culture and Identification Kit
    Mouse Bone Marrow-derived Dendritic Cells (BMDC) Culture and Identification Kit
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    Sku ELSXJM003-20
    Manufact. SkuXJM003-20
    Packaging Unit20 Assays
  8. Mouse Bone Marrow-derived Dendritic Cells (BMDC) Culture and Identification Kit
    Mouse Bone Marrow-derived Dendritic Cells (BMDC) Culture and Identification Kit
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    Sku ELSXJM003-200
    Manufact. SkuXJM003-200
    Packaging Unit200 Assays
  9. Mouse Th17 Flow Cytometry Staining Kit
    Mouse Th17 Flow Cytometry Staining Kit
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    Sku ELSXJM002-20
    Manufact. SkuXJM002-20
    Packaging Unit20 Assays
  10. Mouse Th17 Flow Cytometry Staining Kit
    Mouse Th17 Flow Cytometry Staining Kit
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    Sku ELSXJM002-100
    Manufact. SkuXJM002-100
    Packaging Unit100 Assays
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