Products from BPS Bioscience require a minimum order value above 400€
Applications: Screen small molecule inhibitors in high throughput screening (HTS) applications.
Background: Cathepsin D is a lysosomal aspartyl protease, of the peptidase A1 family. It is involved in lysosomal protein degradation and activation of proteins that are synthesized as precursors, such as hormones and growth factors. Its activity impacts cell death and inflammation. Cathepsin D dysfunction has been linked to breast and gastric cancer, Alzheimer's disease (AD) and neuronal ceroid lipofuscinosis (NCL). Overexpression of this protein can lead to activation of VEGF-C (vascular endothelial growth factor -C) and VEGF-D and metastasis and angiogenesis. Cathepsin D is therefore a promising new therapeutic target. It has been shown that an antibody against Cathepsin D was able to inhibit grow of triple-negative breast cancer cells. Cathepsin D inhibition is also a promising approach in combination treatments, by maximizing the effect of other anti-cancer drugs.
Description: The Cathepsin D Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin D for screening and profiling applications. The Cathepsin D assay kit comes in a convenient 96-well or 384-well format, with enough recombinant human Cathepsin D (amino acids 21-412), its substrate, and Cathepsin buffer for 96 or 400 reactions. This kit contains Pepstatin A as internal control.The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Seo S., et al., 2022 Cell Death and Disease 13: 115.
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