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Encompassing Amino Acids: 207-327
Description: Recombinant human vascular endothelial growth factor (VEGF)165 expressed in CHO cells. Human VEGF exists in multiple molecular variants and are named based on the number of amino acids they contain. VEGF165 is the most common form of VEGF.
Endotoxin Level: Endotoxin level was found to be < 0.1 ng/g (1EU/g), using the LAL gel clot method.
Format: lyophilized protein
Formulation: Recombinant VEGF lyophilized from 0.2 µm filtered 20 mM PB solution, pH 7.0.
Genbank: NP_001165097
Host Cell Line: CHO
Purity: >95%, as determined by SDSPAGE and HPLC.
Reconstitution: Briefly centrifuge the vial, followed by reconstitution in distilled water to a concentration of ≥0.1 mg/ml. This solution can then be further diluted into other buffers containing carrier protein (0.1% BSA).
Storage Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at 2 - 8° C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Uniprot: P15692-4
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Reproduction. 2009; 138: 667-677.
2. Ann. Onc. 2009; 20: 1639-1646.
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