Analysis of 3D cell cultures

Corning Cell Counter

Traditionally, Corning’s cloud-based automated cell counter has focused on rapidly capturing the detailed-analysis of 2D cell cultures. The addition of the organoid counting application expands our cell counting capabilities into the vastly developing 3D space. The robust organoid counting software package is designed to meet the unique needs of customers as 3D populations are known to vary in morphologies dependent on numerous environmental factors or protocols. Engineered to account for complex shapes and sizes, the organoid counting software introduces two versions of the 3D counting algorithm.

Image captured using V2
Image captured using V1

Version 1 (V1) is for use with cultures of irregular morphology. The machine learning algorithm for V1 is trained to engulf the individual outer membranes within the field of view in order to calculate accurate surface area and orgs./mL data.

Version 2 (V2) of the 3D algorithm is for use with spherical objects. Other than obtaining prompt surface area and orgs./mL data; V2 will be optimal for differentiating individual spherical objects within clusters.

Features and benefits:

  • Accurate – powered by machine learning artificial intelligence

  • Multi-informative – organoids per milliliter of solution and surface area data

  • Fast – three-second analysis utilizing cloud-based processing

  • Cost-efficient and versatile – compatible with a standard 0.1 mm counting chambers and 0.2 mm counting chambers. No consumables required.


  • Organoid size range 20 μm to 100/200 μm, limits depend on counting chamber

  • Measurement time <3 sec, depending on up- and download speed

  • Compatibility: Reusable and disposable counting chambers with height: 0,1 or 0,2 mm

  • Sample volume: 10 μL or 20 µL chamber permitting

  • Field of view: 1,5 x 1,5 mm

  • Magnification: 100X

  • Image resolution: 1,536 x 1,536

  • Algorithm Version: Version 1 irregular morphology, Version 2 spherical objects

Flyer Organoid Software

Application Note Organoid Software

Ordering information:

Cat. No.




Corning® Organoid Counting Software w 0.2mm Counting Chamber



Corning® 0.2mm Counting Chamber for Organoid and Spheroid measure, non-grid


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More information about the Corning Cell Counter can be found here.